This study aimed to examine the effects of open and closed kinetic chain exercise interventions and to determine how these structured training programs influence selected physiological variables, specifically breathe holding time and VO2 Max, among inter-collegiate cricketers. A total of 45 inter-collegiate male cricketers aged 18–25 years from AAA College of Engineering and Technology, Amathur, Sivakasi, Tamilnadu were selected for the study. The participants were randomly assigned into three groups: An Open Kinetic Chain Exercise Group (OKCEG), a Closed Kinetic Chain Exercise Group (CKCEG), and a Control Group (CG). The experimental groups underwent their respective structured kinetic chain training programs for six weeks, while the control group continued with their regular physical activities. Breathe holding time and VO2 Max was selected as the dependent physiological variable and were assessed using a standardized test. Pre- and post-intervention data were statistically analyzed using appropriate inferential statistical techniques to determine significant differences among the groups. The level of significance was set at p < 0.05. The results revealed significant improvements in breathe holding time and VO2 Max in both the open and closed kinetic chain exercise groups when compared to the control group (p < 0.05). The control group did not exhibit any significant change in breathe holding time and VO2 Max. The findings of the study indicate that structured open and closed kinetic chain exercise interventions are effective in enhancing breathe holding time and VO2 Max among inter-collegiate cricket players. Both training methods proved superior to regular activity alone, highlighting the importance of incorporating kinetic chain exercises into cricket training programs to improve physiological performance.