This study investigated the hepatoprotective effects of plantain meal on liver enzyme levels in ethanol-induced Wistar rats. Eighteen male Wistar rats (160–270 g) were divided into three groups: Group I (control), received standard rodent chow; Group II, was fed a mixture of plantain meal and normal feed at a 1:1 ratio; and Group III, received a 2:1 plantain-to-feed ratio. After 14 days of dietary intervention, all groups were induced with 80% ethanol (5 ml/kg). Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were measured. The results indicated that plantain meal significantly reduced ALT, AST, and ALP levels in the experimental groups compared to the control, with the 1:1 ratio being more effective. These findings suggest that plantain meal possesses hepatoprotective properties, making it a potential candidate for dietary interventions aimed at liver health.