Background: Postpartum depression (PDD) is a psychological condition that commonly occurs after childbirth and can adversely affect maternal health and child development. Factors such as education level and family support may influence the risk of postpartum depression. Education affects a mother’s ability to cope with emotional stress, while family support is essential for maintaining psychological well-being. Aim: To determine the relationship between education level and family support with the risk of postpartum depression in Kupang City. Methods: This quantitative cross-sectional study involved 70 postpartum mothers selected through purposive sampling from the working areas of Oebobo and Sikumana Public Health Centers. The Edinburgh Postnatal Depression Scale (EPDS) and Postpartum Social Support Questionnaire (PSSQ) were used. Data were analyzed using univariate, bivariate (Chi-Square), and multivariate (binary logistic regression) analyses. Results: Most respondents had secondary education (50%) and good family support (81.4%). No significant association was found between education level and Postpartum depression risk (p=0.801). Family support showed a significant association with PPD risk (p<0.001). Multivariate analysis indicated family support as the dominant factor (p=0.001; Exp(B)=12.039), while education level was not significant (p=0.315; Exp(B)=1.530). Conclusion: Education level was not associated with PPD risk, whereas family support was significantly associated and served as a dominant protective factor.