The current project is based on the experimental work of Dr, Ashwani Kumar. Drug tolerance and resistance are the major proponents of any bacterial disease like TB. These mechanisms are achieved through unique phenomenons like the formation of a biofilm. The current project focuses on the formation of biofilms in Mycobacterium smegmatis (Msm) cultures. These biofilms are in the form of pellicles. The principal work done in the current project is the induction of biofilm using DTT at a range of concentrations in a 96 well plate. The films were then visualised using the Crystal Violet assay. The entire project focuses more on the process of biofilm formation and the mechanisms. The performed work can be further used for screening drugs and exploring ways to combat AMR.