Lutein has a range of nutritional and health-enhancing characteristics, based on its multifaceted biological action in people, which comprise antioxidative, immunomodulatory, and anti-inflammatory properties. This study explored the effect of repeated of lutein on cerebral antioxidants (Superoxide Dismutase (SOD), Glutathione (GSH)) in the animals that were subjected to memory impairment by Diazepam. Thirty (30) Wistar rats was used for this study and the rats were acclimatized for a period of 14 days, and was then divided into six groups; Group 1: Control, Group 2: Diazepam Only (5mg/kg) , Group 3: Diazepam + Lutein (20mg/kg) , Group 4: Diazepam + Lutein (40mg/kg), Group 5: Diazepam + Lutein (60mg/kg), Group 6: Diazepam + Donpenzil (Standard Drug). Administration was done for a period of 21 days. Diazepam significantly disrupted working memory, spatial learning, and retention, which were reflected by a decrease in spontaneous alternation in the Y-maze and longer escape latencies and increased errors in the Barnes maze. It also lowered SOD and GSH activities in the brain. These deficits were being restored by lutein treatment in a dose-dependent manner, restoring spontaneous alternation, shortening escape latency and error rates, and increasing retention performance. Biochemically, lutein had significant restorative effect on brain SOD and GSH levels which were comparable to donepezil. This research concluded that lutein can mitigate diazepam-induced memory impairment by boosting antioxidant levels in a dose-dependent manner, with medium to high doses being particularly effective. These findings support lutein’s potential as a dietary neuroprotective agent against drug-induced cognitive impairment.