Background: Pre-eclampsia and eclampsia remain significant causes of maternal and perinatal morbidity and mortality, particularly in developing countries. Emerging evidence suggests that micronutrient deficiencies, such as Vitamin D deficiency, may play an important role in their pathogenesis. Understanding the demographic, obstetric, and biochemical differences among healthy, pre-eclamptic, and eclamptic women can help identify risk factors and improve preventive strategies. Objective: To evaluate demographic and obstetric variables, blood pressure profiles, and serum Vitamin D levels among healthy, pre-eclamptic, and eclamptic pregnant women. Methods: This cross-sectional comparative study was conducted among 90 pregnant women attending the Department of Obstetrics and Gynaecology of a tertiary care hospital in Bangladesh. The study population was divided into three groups: healthy pregnant women (n = 30), pre-eclamptic women (n = 30), and eclamptic women (n = 30). Data were collected on demographic and obstetric characteristics, systolic and diastolic blood pressure. Statistical analysis was performed using appropriate tests, and p < 0.05 was considered significant. Results: Pre-eclamptic and eclamptic patients were younger, more likely to be primigravida, and had a lower socioeconomic and educational status compared to healthy controls (p < 0.05). Mean systolic and diastolic blood pressures were significantly higher in pre-eclamptic and eclamptic groups (p < 0.001). Serum Vitamin D levels were markedly lower among pre-eclamptic and eclamptic patients compared to healthy pregnant women, and the difference was statistically significant (p < 0.001). Conclusion: Pre-eclampsia and eclampsia are associated with younger maternal age, primigravidity, and low socioeconomic background. Significantly lower Vitamin D levels among affected women suggest that Vitamin D deficiency may contribute to the development or severity of these hypertensive disorders in pregnancy. Screening and supplementation strategies could therefore play a role in prevention and improved maternal outcomes.