The supply and bioavailability of mineral elements in heavily weathered soils are limited and continuously declining, leading to low agricultural production; hence the need to find sustainable alternatives to maintain the fertility of various soils. The objective of this study was to evaluate the change in the chemical properties of soils induced by the addition of Bradyrhizobium japonicum in soybean cultivation across three types of soil in the Haut-Katanga province. Two soil samplings were conducted to carry out this study. The first was done before the start of the trial; and the second during the experimental phase at the flowering stage of the soybeans. This experiment was conducted using a split-plot design, replicated three times. The collected soil samples were subjected to chemical analyzes. According to the results obtained, the changes in chemical properties were significantly different between the soil types. On one hand, a negative change was observed in the eutric Cambisol, namely a decrease in organic matter, total nitrogen, available and total phosphorus, as well as magnesium; on the other hand, a positive change was observed in the two Ferralsols (acric and xanthic), namely an increase in several chemical properties, except for ammoniacal nitrogen which decreased. Regardless of the soils, the addition of external strains led to a decrease in available phosphorus, while only the USDA 142 strain induced similar effects to the control on the increase of K as well as CEC. This has demonstrated the importance of soybean inoculation with Bradyrhizobium japonicum strains as an alternative option to improve crop productivity in acidic soils.