Aim: The aim of this observational study was to evaluate the relationship between neck pain and fatigue levels among individuals engaged in desk-based occupations. Methodology: Neck pain–related disability was assessed using the Neck Disability Index (NDI). Fatigue levels were measured using the Fatigue Severity Scale (FSS) and the Visual Analogue Fatigue Scale (VAFS). A total of 20 office-based workers (13 females, 7 males) participated in the study. The mean age of the sample was 31.30 years. The mean height was 1.64 ± 7.6 m, and the mean weight was 63.4 ± 9.8 kg. Participants completed the NDI, FSS, and VAFS questionnaires through a survey method after providing written informed consent. Results: A statistically significant negative correlation was found between NDI and VAFS scores (Pearson’s r = –0.45421, p = 0.0037). Higher neck disability was associated with greater fatigue levels. Mean scores for individual FSS items ranged from 2.76 to 4.37. Conclusion: The study demonstrated that greater neck disability is associated with increased fatigue among desk-job workers. These findings highlight the importance of posture correction, ergonomic modifications, and early screening to reduce neck-related disability and fatigue in individuals engaged in prolonged sitting occupations.