Background: Understanding the perception of attractiveness in the chin position relative to the aesthetic level of the E-line is crucial for improving aesthetic outcomes in orthodontic. This study aimed to examine the extent to which changes in the chin position at sagittal plane relative to the E-line are observed in terms of attractiveness among laypeople, dentists and orthodontists. Material and methods: A digital portrait of adult woman was generated by artificial intelligence (AI) for the study. The image was digitally altered using Adobe Photoshop to create 3 images and presented to 60 orthodontists and 60 laypeople for evaluation of their perception of facial profile attractiveness on a visual value rating scale of (1 to 5). Results: The study results showed that a repeat measures ANOVA was conducted to examine the effect of chin position profile in relation to the E-line (based on three images) on facial attractiveness ratings, and whether this effect differed between orthodontists and laypeople. However, there was a significant interaction between image type and participant group (Wilks' Lambda Test). Conclusions: Orthodontists and Laypeople rated the retrusive chin as more attractive than the protrusive of the chin. Future research should further explore how demographic and cultural factors influence these aesthetic judgments.