Background: Gender inequality in the nursing workforce is still a big issue, especially in rural areas such as Osmanabad, Maharashtra. Even though nursing is a profession dominated by women, women are still confronting systemic obstacles to leadership, to pay equity, and to a safe workplace. These differences are exacerbated by cultural standards, restricted institutional assistance, and a lack of resources in rural health care facilities. Objectives: Considering the background, this study is planned to look into the magnitude of gender inequality of nurses in the district of Osmanabad. More specifically, these aims will: identify gender differences in recruiting, role assignment, and career advancement; measure acts of violence and the effect of perceptions of unsafe work settings; provide practical strategies to move toward gender equity within rural nursing. Methods: The present study was conducted using a mixed methods approach with both quantitative and qualitative data generation. Stratified purposive sampling was employed to select a sample of 60 registered nurses working at PHCs, CHCs, and the District Hospital, Osmanabad. Information was collected using questionnaires, semi-structured interviews, and focus group discussions. The quantitative data were processed with SPSS, and the qualitative data were subjected to thematic analysis. Results: Female nurses were predominantly represented, although not in leadership roles, and female nurses were significantly underpaid compared with their male colleagues. Women experienced workplace violence more often compared to night shifts. Emotional exhaustion, professional inertia, and a wish for change were depicted in qualitative accounts. Conclusions: Sex disparities in the nursing workforce existed in Osmanabad and are indicative of wider systemic imbalances in rural health care. Overcoming these inequalities through enabling policies, mentorship programs, and community engagement is crucial in strengthening nursing and healthcare. The report highlights the requirement for gender transformative interventions suitable for rural areas.