It is a general practice to engage in waterbodies with anthropogenic activities, which can alter the physicochemical nature of the water and introduce various types of pollutants. Several types of pollutants are responsible for inducing oxidative stress in aquatic organisms, leading to tissue and nucleic acid damage, and ultimately resulting in significant mortality and economic losses to aquaculture. In the present study, the physicochemical parameters of the anthropogenically involved pond were analyzed for all four seasons (summer, monsoon, post-monsoon, and winter). The oxidative stress (DPPH, FRAP, and H202) in two major carps (Labeo rohita and Catla catla) was studied, and the results of oxidative stress in the form of micronucleus development were analyzed to establish the mortality of carps. The lower alterations in physicochemical concentrations were reported in the monsoon season and were maximum in the winter season. The oxidative stress was also reported as maximum in winter and minimum in the monsoon season in both carp. Relative micronuclei have also been reported to be comparatively higher in C. catla than the L. rohita. The findings of the study provide substantive pieces of information not only for the effect of pollutants on fish but also give an idea of the suitability of species for aquaculture.