The chlorophyll a (Chl. a) and b (Chl. b) and carotenoid contents of 10 distinct medicinal plants have been determined in the current investigation. Young and mature leaves were found to have different chlorophyll a (Chl.a) and b (Chl.b) and carotenoid contents in terms of quality. For the purpose of evaluating the chlorophyll and carotenoid concentration, 10 different medicinal plant species were chosen, including Pterocarpus santalinus, Azadirachta indica, Phyllanthus emblica, Tridox procumbens, Swietenia mahagoni, Hardwickia binata, Pithecetobium dulce, Cassia fistula, Dalbergia latifolia and Syzygium cumini. In all cases, adult leaves had more chlorophyll and carotenoid content than young ones. It has been demonstrated that the age of the leaves has a significant impact on the amount of chlorophyll and carotenoids.