Background: Menstruation is a universal and normal phenomenon during the reproductive age of females. Adolescent is an essential period where females are preparing and adjusting themselves to manage their menstrual bleeding in safe and clean way. Menstrual hygiene forms a definite part in the life, of the development of the girl’s child in schools and the general development of women of boarder the context. Globally there were 1.2 billion adolescent girls in the year 2009 which forms eighteen percent of the world’s population. In Bangladesh, there are approximately 27.7 million adolescents and 13.7 million girls, one-fifth of the total population. Aim of the study: The aimed was to assess the level of knowledge and practice regarding the menstrual hygiene among adolescent girls at Yakubia Girl’s High School, Bogura. Methods: This was a descriptive cross sectional study design was used and sample size 120 that was simple random sampling technique followed those who meet the inclusion criteria and to assess the knowledge and practice regarding the menstrual hygiene among adolescent girls. The study was conducted from December, 2024 to May, 2025. The instruments for data collection were a semi-structured questionnaire which composed of three parts: Demographic variables, knowledge and practice-based information on menstrual hygiene. Result: The findings of the current study reported that the average knowledge score 60% were moderate and 40% were low level of practice regarding the menstrual hygiene. A possible reason for this could not be the wide availability of water and soap in schools and other facilities. Conclusion: Menstrual hygiene is an essential component of adolescent health, playing a pivotal role in individuals' overall wellbeing and development. The menstrual hygiene practices in Bangladesh can help policymakers and program developers design effective interventions to improve menstrual hygiene, such as promoting education, providing access to affordable hygiene products, and improving sanitation facilities for adolescent girls