Background: The COVID-19 pandemic has placed unprecedented demands on healthcare systems worldwide. Nurses, as frontline caregivers, play a critical role in the management, prevention, and control of COVID-19 infections. Adequate knowledge among nurses is essential to ensure effective patient care and reduce transmission risk in healthcare settings. Aim of the study: This study aimed to assess the knowledge of nurses regarding COVID-19 patient management in a tertiary-level hospital in Bangladesh and to analyze factors associated with their knowledge levels. Methods: A descriptive cross sectional study design was used and sample size 320 that was convenient sampling technique followed those who meet the inclusion criteria and to assess the state of knowledge of nurses in COVID-19 patients’ management in a tertiary level hospital at Faridpur Medical College Hospital, Faridpur. The study was conducted from January 2020 to December, 2020. The instruments for data collection were a semi-structured questionnaire which composed of two parts: Demographic variables, and knowledge on management based information on covid-19. Result: The findings revealed that the highest, 45.3% from 36 –5 years age group and 93.1% nurses were female, in educational qualification were diploma in nursing 86.6 %, and 85.3% were married and 53.8% were less than 5 years working experiences. This study's main findings revealed the average 52% respondent’s knowledge was good, as well as 28% respondent’s knowledge was fair, and 20% respondent’s knowledge was poor about covid-19 management. Regarding the association, the finding was a significance association present between nurse’s work experiences and level of knowledge of nurses on prevention and control of COVID-19 (χ2 = 23.06, P<.003). On the other hand, there were no significance association between nurse’s educational qualification and level of knowledge of nurses on prevention and control of COVID-19 (χ2 = 8.47, P<.076). It’s may due to their clinical experiences. Conclusion: The findings suggested for improving the situation of nurses about health care associated knowledge by training, higher education, seminar and workshop of COVID-19 patient management in a tertiary level hospital and providing comprehensive psychological support to the nurses in order to prevent stress disorder.