A comparison of Automated and manual methods to determine the hemoglobin concentration and Hematocrit was done using a specified sample size of randomly selected patients. All the samples were subjected to Hb and Hematocrit estimation by using both manual and Automated methods. A significant difference between manual and automated Hct mehods was found. There was a good correlation between Cyanmeth-Hb and Automated method. The Cyanmeth-Hb is a gold standard for Hb estimation and also carries a biotoxic hazard. However, there was a statistically significant difference between Sahli’s and cyanmeth- Hb method, but there was a good correlation. The Hct estimation by manual and automated methods showed an excellent relationship and no significant difference was noted between two methods and that can be used interchangeably.