Urinary tract infections (UTIs) are among the most common bacterial infections affecting individuals worldwide, often leading to significant morbidity if untreated. Accurate bacteriological profiling of urinary isolates is crucial for appropriate diagnosis and effective antimicrobial therapy, especially in the face of rising antibiotic resistance. This study aimed to identify the aerobic bacterial pathogens isolated from urine samples and to determine their antibiotic susceptibility patterns in a tertiary care hospital setting. A cross-sectional study was conducted over a period of one year (January 2024 to December 2024), where urine samples from suspected UTI patients were cultured and bacterial isolates were identified using standard microbiological techniques. Antibiotic susceptibility testing was performed following Clinical and Laboratory Standards Institute (CLSI -2024) guidelines using Kirby-Bauer disk diffusion method. The results revealed Escherichia coli as the predominant pathogen, followed by Klebsiella pneumoniae and Pseudomonas aeruginosa. Nitrofurantoin and amikacin showed the highest susceptibility rates, whereas a notable resistance to Fluoroquinolones was observed. These findings underscore the importance of continuous surveillance of urinary pathogens and their resistance profiles to guide empirical treatment and combat the spread of multidrug-resistant organisms in hospital settings. Implementation of antibiotic stewardship programs is imperative to optimize antibiotic use and improve patient outcomes.