Background: Multiple sclerosis is a compound sickness origin via a fundamental interaction compound which is un inheritable factor–environment in addition defined through inflaming toward neurodegenerative the cells of the central nervous system stop working or die demonstration. Alteration the pathologic process and the line of multiple sclerosis due to different molecular modification, which including rises in interleukins, chemokines, NO, species of activated oxygen, self dertermining radicals and glutamate. Material & Methods: This study included 37 patients taking (5 males ,32 females) and 25 healthy controls. Blood samples were obtained from the biological treatment Unit for neurological diseases in Baghdad Teaching Hospital in the period from November 2023 to March 2024. The serum was isolated by centrifuging blood samples at 1000g for 5 minutes. Human TNF-ɑ levels were quantified using an ELISA kit (Elabscience Biotechnology Inc., USA;133Hu, Lot. no. L240214179.Following the manufacturers protocol. Results: The results of the present study showed that patients with M.S. had higher levels of TNF-α 34.33 pg/ml while control group had lower levels of TNF-α 28pg/ml, there were a significant difference between patients, P=0.028. Conclusion: Patients with M.S. had higher levels of TNF-α than their levels in control group, provided a fundamental indication that an increment of TNF-α happen topically inside the central nervous system of patients with M.S.