Background: Cytomegalovirus (CMV), a member of the herpesviridae family, is believed to play a role in the development of cervical carcinoma in women. About 50–85% of people worldwide are infected with CMV by early adulthood. Objective: The purpose of this study was to ascertain the prevalence of CMV in Medani, Gezira State, among women who had cervical cancer. Materials and Methods: The study involved 50 women in total. Participants' blood samples (5 mL each) were drawn and transferred into sterile, plain blood containers. An enzyme-linked immunosorbent assay (ELISA) was used to analyze the serum for CMV IgG and IgM antibodies after it was collected by centrifugation at 3000 g for five minutes (Snibe Maglumi X3). Results: According to the findings, 32 (64%) of the 50 women tested positive for CMV antibodies, while 18 (36%) tested negative. Two (6%) of the 32 positive patients had IgM antibodies, which indicated a recent infection, whereas 30 (94%) had IgG antibodies, which indicated a previous or latent infection. According to the distribution of CMV frequency by age group, older women had a greater frequency: 3% of women aged 20–30, 22% of women aged 31–40, and 75% of women aged 40 and above. Furthermore, women with a managed menstrual cycle had a higher frequency (26%) than those with an uncontrolled cycle (20%). In addition, women who married young were more likely to do so (38%) than those who married later (26%). Conclusions: According to the study's findings, women in Gezira State with cervical cancer had a notably high frequency of CMV, especially those who were older. To confirm these results and investigate the part CMV plays in cervical carcinogenesis, more studies with larger sample numbers and more sophisticated diagnostic methods are advised.