Hormonal contraceptives (HCs) are widely used for birth control. There are reported adverse effects associated with HCs and liver and kidney function in people on hormonal contraceptives. Limited studies exist to establish these reports; it is however crucial to elucidate any potential associations between these medications with liver and kidney dysfunction. This study aims to evaluate the impact of hormonal contraceptives on liver and renal function. For this study, a total of 50 participants were used; 25 of the total participants are women of reproductive age, using hormonal contraceptives, while the other 25 participants are women of reproductive age, not using hormonal contraceptives. The participants were recruited from Orita-Obele and Arakale health centers, Akure, Ondo State. The results showed a significant increase (p<0.001) in both creatinine and urea levels when compared to the control, but no significant increase (p>0.05) in both sodium and potassium ion concentrations. A significant increase (p<0.0001) in aspartate aminotransferase (AST), alanine transferase (ALT) activity, along with a significant increase (p<0.01) in bilirubin level, were observed in the case when compared with the control group. The results suggest that the alterations observed in kidney and liver function of subjects on hormonal contraceptives may indicate potential impairment. Understanding these interactions is crucial for ensuring the safe use of hormonal contraceptives and managing any potential risks to liver and kidney health, thereby guiding clinicians in prescribing these medications and monitoring their effects.