Background: Vitamin D deficiency and dyslipidemia are both common in individuals with type 2 diabetes and may contribute to increased cardiovascular risk. This study aims to assess the correlation between serum 25-hydroxyvitamin D levels and lipid profile parameters in patients with type 2 diabetes mellitus. Aim of the study: The aim of the study was to evaluate the correlation between 25-Hydroxyvitamin D levels and lipid profiles (TG, TC, LDL, HDL) in individuals with Type 2 diabetes. Methods: This cross-sectional study was conducted at the Department of Clinical Biochemistry and the Department of Biochemistry and Cell Biology, BIRDEM General Hospital, Dhaka, Bangladesh, from July 2014 to June 2015, including 200 participants (130 with type 2 diabetes, 70 healthy controls). After informed consent, demographic data and blood samples were collected for fasting plasma glucose, lipid profile, 25-hydroxyvitamin D, HbA1C, and postprandial glucose analysis. Biochemical tests were performed using standard methods, and data were analyzed with SPSS v21, with statistical significance set at p ≤ 0.05. Results: Type 2 diabetics had significantly lower vitamin D levels and higher BMI compared to non-diabetics. Hypovitaminosis-D was more common in diabetics (44.6% vs. 22.9%, p = 0.002). Diabetics also had higher total cholesterol, LDL, and triglycerides (all p < 0.001). In diabetics, vitamin D levels were inversely correlated with cholesterol, LDL, and triglycerides, and positively correlated with HDL. Conclusion: Vitamin D deficiency is significantly associated with dyslipidemia in type 2 diabetic patients, showing strong negative correlations with LDL and triglycerides and a positive correlation with HDL.