This study aims to develop a self- assessment instrument that evaluates the Transition to Practice Cardiac Nursing Program (TTP-CNP) using MEASURE approach in a Tertiary Military Cardiac Specialized Centre in Saudi Arabia. The TTP-CNP instrument’s reliability and validity were evaluated. Cronbach’s Alpha was used to estimate the instrument internal consistency. The construct reliability was measured using test-retest reliability. Content and face validity were measured using content validity ratio (CVR), content validity index (CVI), and face validity index (FVI) and the instrument was determined to be valid and reliable. The instrument can be used as a reliable and valid measure for assessing TTP-CNP programs, however, more testing on larger samples using Exploratory Factor Analysis (EFA) and Confirmatory Factor Analysis (CFA) is suggested to confirm our conclusion.