This study aims to examine the relationship between physical readiness, emotional intelligence, and work discipline and the professional performance of police officers in the Gasum Sub-Directorate of Ditsamapta, Special Region of Yogyakarta. The research employed an ex post facto approach, with a sample of 40 police officers selected using a total sampling technique. The instruments used include: (1) a physical readiness test based on Decree No. Pol: Skep/984/XII/2004 (12-minute run, pull-ups, sit-ups, push-ups, shuttle run); (2) an emotional intelligence questionnaire with a KMO MSA value of 0.760, AVE of 0.760, and reliability of 0.927; (3) a work discipline questionnaire with an average t-value of 8.33 and reliability of 0.981; and (4) a professional performance questionnaire with factor loadings ≥ 0.40 and reliability of 0.70. Data were analyzed using prerequisite tests (normality and homogeneity) and multiple linear regression analysis. The results show that: (1) there is a very strong and significant relationship between physical readiness and professional performance (R = 0.923; R² = 0.852); (2) emotional intelligence also has a very strong and significant relationship with professional performance (R = 0.971; R² = 0.943); (3) work discipline has a very strong relationship with professional performance (R = 0.984; R² = 0.968); and (4) collectively, the three variables have a significant relationship with professional performance (R = 0.989; R² = 0.978). It can be concluded that physical readiness, emotional intelligence, and work discipline are significantly related to professional performance, both individually and simultaneously.