Objective: To find out the association between atherogenic index and thyroid status in hypothyroid patients. Method: This case control study was conducted in the Biochemistry Department of National Institute of Kidney Diseases and Urology during the period of 2018 and 2019. 100 subjects aged between 20-50 were selected by purposive sampling from the patients and attendants attending to the outpatient department of NIKDU. Among them 55 subjects were diagnosed case of hypothyroidism with TSH >4ng/dl and FT4<0.9 ng/dl and 45 subjects were age and gender matched healthy euthyroid controls. Here student unpaired t-test, Chi-square test and Pearson’s correlation test were used to determine the association of atherogenic index and hypothyroidism. SPSS version 23 was used for the level of significance and p value <0.05 was considered statistically significant. Result: A total 100 subjects were included in the study without subjects with hypertension, diabetes mellitus and renal failure. All the variables were compared between the two groups. Out of 55 cases, 20 were males and 35 were females. Out of 45 control subjects, 12 were males and 35 were females. There was no statistically significant difference in case and control group in term of gender and Chi-square test was done to measure the level of significance. Among case and control, highest percentage were in the range of 20-30 years with 43.6% and 55.5% respectively. The mean age of case group was higher than the control group, but the difference was not statistically significant (p<0.05). Mean AIP level was 0.75±0.27 in case group and 0.35±0.20 in control group and was statistically significance difference (<0.05) between the two groups. Conclusions: From this study, it may be concluded that high atherogenic index is associated with Hypothyroidism. Hypothyroid patient with increased AIP is prone to develop cardiovascular diseases. So, to decrease the incidence of cardiovascular diseases in hypothyroid individuals, regular monitoring of serum lipid profile should be done by clinician.