Sclerocarya birrea tree (Marula), is known in Sudan as Homeid tree. The different parts of the tree find wide traditional and medicinal uses. This study was carried to evaluate the chemical composition of the fruit seeds and kernels oil from nutritional value sight of view. Fresh fruits were collected from Darfur and Kordofan States. The proximate constituents of seeds were determined. Kernels oil was extracted by Petroleum ether. The physicochemical properties of the oil were measured. Seed kernels content of some minerals was determined by AAS. The extracted oil was analyzed by GC-MS. The proximate seed constituents were ash (4.93%), moisture (5.74%), pH (6.36%), protein (44.17%), vitamin C (19.47mg/100g) and Fiber (5.32%). The AAS analysis showed high P (220.25mg/100g) and K (86.10mg/100g) in the kernels. The oil yield was (53.47%). The physicochemical properties were acid value (0.42mg KOH/g), Peroxide value (4.64meq/kg), Saponification value (199mg KOH/g), viscosity (62.1), refractive index (1.467), density (0.919g/cm3) and un-saponified matter (3.17%). The GC-MS characterization showed a presence of five constituents, Dominated by Oleic (65.97%), Palmitic (17.53%) and Stearic acid (12.84%).