This study aimed to explore the gender and hierarchical level differences in loneliness at work. A sample of 143 employees who worked at the Thai government bank headquarters was gathered by using proportional stratified sampling technique. Loneliness at Work Scale (LWS) was modified to use as the instrument for data collection. Results of independent samples t-test analysis revealed that there was a significant difference in males and females with respect to loneliness at work (t = 3.079, p < 0.002). This suggested that males were experiencing loneliness at work more than females. Results also showed a significant difference in employees and employers with respect to loneliness at work (t = -3.458, p < 0.001). This suggested that employees were experiencing loneliness at work less than employers. This present study suggested that the further study should compare mean differences of loneliness in the workplace in other sociodemographic variables.