Background: Many secondary metabolites found in plants, including tannins, terpenoids, alkaloids, flavonoids, glycosides, etc., have been shown to exhibit antibacterial activities in vitro. Man has long been aware of herbal treatments. Traditional medical practitioners have detailed the therapeutic usefulness of numerous indigenous herbs for a variety of illnesses. More and more reports of medicinal plants' antimicrobial qualities are coming in from all over the world. By producing secondary metabolites with antibacterial characteristics, these plants offer another option for creating chemical fungicides that are both reasonably safe and reasonably priced. The Lamiaceae family member Coleus aromaticus is bitter, aromatic, digestively stimulating, stomachic, anathematic, deodorant, diuretic, and liver-tonic. Aim: The aim of the study is to assess the antimicrobial activity and to determine the zone of inhibition of extracts on some fungal strains. In the present study, the microbial activity of hydroalcoholic and methanolic extracts of leaves of Coleus aromaticus Linn. (an ethnomedicinal plant) was evaluated for potential antimicrobial activity against medically important fungal strains. Method: The antimicrobial activity was determined in the extracts using agar disc diffusion method. Result: The zone of inhibition for different strains of fungia i.e. CA, CA, AN were determined. The outcome of the investigation revealed that hydroalcoholic leaf extract of CA is more effective than methanolic extract as compare to standard due to presence of flavonoids. Conclusion: Both extracts (MeoH & HAE) showed effective antifungal activity, of which the HAE demonstrated the potent antifungal activity due to high flavonoid content.