The study assessed the impact of Odogwu bitters on biochemical indices of rats. In this experimental study, 16 adult male rats were used. The rats were randomly divided into four (4) groups. Groups 1-3 were given 100, 200, and 400mg/kg of Odogwu bitters. Group 4 served as the normal control. Serum liver enzyme activity, kidney function enzyme activity, Lipid profile test and haematology tests were carried out. The collected data were analyzed using SPSS, ANOVA, and LSD tests. Elevated levels of ALT and AST in rat plasma indicated potential liver tissue stress due to herbal exposure, aligning with findings from similar herbal studies. However, at higher doses, a reduction in these enzyme levels was observed. Conversely, ALP levels increased significantly with the highest dosage, suggesting potential liver damage. Elevated levels of urea, creatinine, and uric acid in the treated groups indicate potential adverse effects on renal function. Urea, vital for waste nitrogen transport and maintaining osmolarity, showed significant alterations, suggesting potential kidney stress. Similarly, increased creatinine levels, a marker of muscle metabolism and renal health, imply compromised kidney filtration. The disturbance in uric acid metabolism, crucial for various physiological functions, points towards potential renal impairment. Haematological results showed a concentration-dependent increase in hemoglobin levels, potentially supporting hemoglobin synthesis. Platelet counts exhibited a dose-related decrease, suggesting an influence on platelet production or function. Red blood cell levels were maintained, possibly due to antioxidant compounds protecting against oxidative damage and stimulating blood cell production. White blood cell levels remained steady, indicating potential immune-boosting effects. Different doses of Odogwu herbal bitters influenced lipid metabolism in Wistar albino rats, with Group 2 showing a reduction in TG levels and Group 3 having the lowest total cholesterol (TCHO). Groups 2 and 3 also had increased HDL levels, while the control group exhibited the lowest LDL levels, suggesting varied effects of the bitters on lipid profiles. The findings collectively suggest a detrimental impact of Odogwu bitters on kidney indices in the studied animal model and could be toxic at a higher dose upon consumption on the liver. But for the lipid status, Odogwu herbal bitters may influence lipid profiles, their effects are not uniformly beneficial across all parameters.