Aim: To analyze the mental foramen dimensions and location for gender determination in a sample of Yemeni population. Methods: This is a retrospective study performed on 420 maxillofacial CBCT scans, 210 males and 210 females with age ranged from 20 to 60- years old. Osteometric analysis of the dimensions, shape and position of the mental foramen was performed. Results: In comparison between males and females, all mental foramen (MF) measurements were statistically significant except at the distance from the superior mental foramen to the alveolar crest. The all measurements of males were higher than females. MF with round shape was the most common between both males and females. The most common location of the MF was presented below the apices of the 2nd premolars. Conclusions: We can be concluded that the vertical and horizontal dimensions the MF and the distance from the lower border of the MF to the lower border of the mandible exhibits gender dimorphism in the Yemeni population. The shape and horizontal position of the MF do not show any difference denoting that they cannot be used for identification of gender.