Background: Preventive dentistry plays a crucial role in maintaining optimal oral health and preventing dental diseases such as caries, periodontal disease, and oral cancer. Knowledge and awareness of preventive measures significantly influence patient behavior, improving oral health outcomes. This study aimed to assess the awareness of preventive dentistry practices among patients attending Karary University Dental Clinic in 2022. Methods: A descriptive cross-sectional study was conducted with 151 patients attending the dental clinics at Karary University. A structured questionnaire was used to evaluate participants' knowledge and attitudes regarding preventive dentistry practices. Descriptive and inferential statistical methods were employed for data analysis. Results: The majority of participants (54.7%) exhibited fair awareness of preventive dentistry, with 39.3% showing good awareness, 4.7% demonstrating poor awareness, and only 1.3% displaying excellent awareness. Awareness was significantly higher among females, individuals aged 19-40 years, and those with a university education. While most patients (90.4%) recognized the preventability of dental diseases, gaps in knowledge were evident regarding the use of preventive tools such as dental floss and the importance of regular dental checkups. Conclusion: This study highlights a significant variation in preventive dentistry awareness among patients at Karary University Dental Clinic. Despite general awareness of dental disease prevention, specific areas, such as the use of dental floss and understanding advanced preventive measures, require further attention. Targeted educational interventions, particularly for high-risk groups, are essential to improve preventive dental practices and ultimately enhance oral health outcomes.