Aim: To assess the dental treatment anxiety levels & oral health status among university students of different health occupations in Pune city, & to correlate it with their fields of study & between the genders. Materials and Methods: A cross-sectional study was conducted among 293 university students from Physiotherapy, Pharmacy, and Unani medicine colleges in Pune city. Data was collected using the Modified Dental Anxiety Scale (MDAS) given by Humphries et al., the modified WHO ’s Oral Health Assessment form (2013) and Oral Hygiene Index-Simplified given by Greene and Vermillion (1964). Descriptive statistics was used to analyze the relationship between anxiety levels, oral health and Field of Study. Results: Pharmacy students had the highest anxiety (mean MDAS score of 15 ± 4.3), followed by Physiotherapy students (14 ± 4.4) and Unani medicine students (13 ± 3.4). Most of the participants had fair oral hygiene(p<0.0001) & majority of them required prompt dental treatment (p<0.001). A statistically significant correlation was found between higher dental treatment anxiety scores and poorer oral hygiene (p < 0.001). Females reported higher anxiety levels than males (p<0.01). Conclusion: Dental treatment anxiety is prevalent among students, with higher levels of dental treatment anxiety correlating to poorer oral health. Interventions targeting dental treatment anxiety reduction and dental health education are needed, particularly for students outside of healthcare fields to provide good dental care.