Background: Central line-associated bloodstream infections (CLABSIs) are significant healthcare challenges, associated with increased morbidity, mortality, and healthcare costs (Magill et al., 2014). Nursing care plays a crucial role in preventing these infections. Objective: This study investigates the impact of nursing interventions on central line care and CLABSI rates in patients admitted to Imam Abdulrahman Bin Faisal Hospital, Dammam, Saudi Arabia. Method: A prospective observational study was conducted from June 2018 to June 2019 with a sample size of 100 patients with central lines. Nursing interventions included daily line assessments, sterile dressing changes, and strict adherence to hand hygiene protocols. Data on CLABSI incidence were collected and analyzed to determine the association between nursing care quality and infection rates. Result: Among 100 patients, 15 developed CLABSIs, yielding a 15% infection rate. Patients with consistent nursing interventions had a 7% infection rate (3 out of 45), compared to 28% (12 out of 55) among those with inconsistent care, reflecting a 75% reduction with effective nursing practices (p < 0.05). Standardized dressing changes lowered infection rates by 40%, from 20% to 12%. Adherence to hand hygiene further reduced infections by 33%, from 15% to 10%. Units with nurse-patient ratios below 1:5 saw a 5% infection rate, significantly lower than the 18% in units with higher ratios, showing a 72% reduction. Conclusions: Effective nursing interventions significantly reduce CLABSI rates, underscoring the need for stringent infection control protocols and adequate nurse staffing. Enhanced nursing training and policy standardization are recommended to further minimize CLABSIs.