The study aims to investigate the mediating role of artificial intelligence technical skill (AI) on the relationship between effectiveness of management information system (MIS) and knowledge acquisition (KA) in Jazan University, the study used descriptive and analysis methods, A questionnaires used for data collection, (229) questionnaires were distributed, (177) valid questionnaires are returned about (%77.29) of the sample size, Several statistical methods have been used. The study found that there is positive and significant relationship between MIS effectiveness and AI technical skills, the study found that AI technical skill mediating the relationship between effectiveness of MIS and KA acquisition. These findings demonstrate the importance of AI in driving the effectiveness of KA. For future, the study recommends to apply difference dimensions of AI with difference dimensions of KA in other sectors.