Anthocleista vogelii (Loganiaceae) is a plant used in traditional medicine in the Cameroon, Ghana, Togo, Nigeria and Guinea for the treatment diabetes. Hallea stipulosa (Rubuaceae) is a plant used in traditional medicine for the treatment of several illnesses such as hernia, metrorrhagia and dysmenorrhea, colic, psychosomatic disorder and diabetes. This study aims to determine the level of toxicity following the protocol of OECD guideline 423 after phytochemical analysis by using screening, High performance thin layer chromatography (HPTLC) and quantitative analysis of the extract’s total polyphenol contents (TPC) and total flavonoids contents (TFTs)were done using folin-ciocalteu and aluminiun trichloride test respectively. The evaluation of the hypoglycaemic activity and a hypoglycaemic activity of the aqueous extracts of the bark of Haleas stipulosa and Anthocleista vogelii. Combined at the concentrations 250mg / kg and 500mg / kg each at the respective proportions 50/50; 30/70; 70/30 using as a method the measurement of blood sugar every 30min for 3 hours. We obtained a yield of 11.65 for the extract of Hallea stipulosa and 10.2 for the extract of Anthocleista vogelii. The analysis of the phytochemical screening revealed the presence of tannins, flavonoids, antocyanins and terpenoids, alkanoids and phenols. The Haleas stipulosa and Anthocleista vogelii fractions have a relatively high TPC of 95,78 ± 0,02 (mg EAG/g de ES), 169,66 ± 0,03 (mg EAG/g de ES), respectively and the TFTs of 62,93 ± 0,06 (mg EQ/g d'ES), 73,45 ± 0,05 (mg EQ/g d'ES) respectively. Acute toxicity tests in female rats at doses of 2000mg / kg and 5000mg / kg revealed no behavioral disturbances and no death. The administration of the combination of the aqueous extracts of the bark of the trunks of Hallea stipulosa at the concentrations of 250 mg / kg BW and 500 mg / kg BW produced a significant hypoglycaemic effect (P ˂0.05) in rats compared to the batches of rats positive controls. These results suggest that the combination of the aqueous extracts of the bark of the trunks of Haléas stipulosa and Anthocleista vogelii exhibits hypoglycemic and antihyperglycemic activity at certaines concentrations.