Background: H. pylori is a micro-aerophilic, a gram-negative, spiral shaped pathogenic bacterium that specifically colonizes on the gastric epithelium and it is one of the most common human bacterial pathogens in wide world. Type 2 Diabetes Mellitus is known as non-insulin-dependent diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia associated with disturbances of the various metabolisms, in particular lipid, carbohydrate and protein. Materials and Methods: In this study, we aimed to determine the profile of lipid markers in T2DM and non-diabetics patients during Helicobacter pylori infection. We carried out a descriptive cross-sectional study over a period from June to November, 2021. Ninety patients were selected divided into two groups, each subdivided into two subgroups. 44 type two diabetes of mellitus patients and 46 non-diabetic patients were included. Result: In ninety patients, the average age of diabetic patients was 51 ± 11 years old. The average age of non-diabetic patients was 40±15 years old. Out of 90 patients in our study population, we had a predominance of women compared to men and infected women represented 76.47% and men 23.52% of the overall population. H. pylori infection was more common in diabetics compared to non-diabetics. In this current study we have observed the disturbance of the lipid profile to variable degrees in diabetics and non-diabetic patients whose H.pylori infection was positive with p-values less than 0.005. The univariate correlation between H.pylori infection and lipid markers showed that H. pylori were associated with abnormalities including cholesterol LDL, cholesterol HDL and triglycerides in our study population. Conclusion: According to Our study H.pylori infection was linked with disturbances of lipid markers and the univariate showed that the H. pylori was susceptible to increase and to fall down lipid profile.