Canola is an important crop with low levels of fatty acid and genetically produced from rapeseed. Canola oil is obtained by extract sources. Heavy metals are a unique form of toxin because they may exist in the environment for a longer period. Exogenous administration of gibberellic acid has been proven in several studies to increase crop output by affecting an important physiological process. This study of the aimed and objectives included the nickel sulphate stress in quantity 300 µM and gibberellic acid in quantity 0.25 mM in specific quantity and their effects on different plant functions. Super canola under stress condition when 0.25 mM GA3 was applied. Whereas, minimum reduction was observed in cv. Sherilla under stress condition when 0 mM GA3 was applied. Maximum Catalase was observed in cv. Results showed the Peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) are the most important protective enzyme systems in plants, and also the most important reactive oxygen species clearance system. Maximum Total Amino acid was observed in cv. Sherilla under control condition when 0 mM GA3 was applied. However, effect of GA3 application was observed non-significant and same for as varietal difference among two genotypes was observed non-significant. Overall, super canola performed better under stress or non-stress condition. Mustard plants exposed to nickel showed decreased morphological characteristics, which might be related to the degradation of plant architecture, which limits the capacity of water content and minerals absorption by plants, resulting in total development of plant loss.