Aim-The aim of minimally invasive biological treatment in endodontics is to effectively manage pulpal and Peri apical pathologies while preserving tooth structure, promoting healing, and enhancing patient comfort. Minimally invasive biological treatment in endodontics represents a contemporary approach expected at preserving tooth structure, promoting healing, and enhancing patient comfort. This treatment philosophy emphasizes conservative interventions that minimize trauma to the tooth and surrounding tissues while harnessing the body's natural healing mechanisms. Key principles include selective removal of diseased tissue, biocompatible disinfection, gentle instrumentation, and the use of biomimetic materials for obturation. By adhering to these principles, clinicians can achieve successful outcomes while preserving tooth vitality and promoting long-term dental health. This abstract explores the principles, techniques, and clinical considerations associated with minimally invasive biological treatment in endodontic, highlighting its benefits for both patients and practitioners.