This study aims to analyze the effectiveness of temephos against Aedes aegypti larvae in the Buffer Airport and Poso Sea Port area. This type of research is quasi-experimental. The research sample used the first-generation instar Fed 1 Aedes aegypti larvae obtained from mosquito breeding. A total sample of 240 tails consisted of treatment and control with 3 replications each. Testing uses 1% temephos concentration. The statistical test used in univariate and probit analysis. Based on the results of the study showed that the Poso Airport Buffer, Aedes aegypti test larvae were susceptible to temephos 1% with a total mortality of 100% of the test larvae. where the WHO standard criteria state for larvae of the test 98-100% Vulnerable, 80-98% Tolerant deaths, and <80% resistant. Likewise, with the Poso Port Buffer region, the Aedes aegypti test larvae are susceptible to temephos 1% with a total of 100% average adult mosquito mortality. the overall lethal time values (LT50, LT90, LT95, LT99) in the Poso Sea Port Buffer region are higher than in the Poso Airport Buffer area, where the Poso Sea Harbor Buffer area for 1% temephos requires a longer time to kill the Aedes aegypti test larvae, compared to the Poso Sea Port Buffer region. by poso airport. Especially to kill 99% of test larvae in the Poso Sea Port Buffer region, temephos 1% takes 147.2 minutes or 2 hours 27 minutes while for the Poso Airport Buffer region it only takes 119.86 minutes or 1 hour 59 minutes to kill 99% of larvae test.
July 26, 2020
An Immense Detail of miRNA Role in Gene Regulation and Their Expression on Functional Bases in Various Crops
Junaid Ali, Muhammad Umer Khan, Hasham Feroz Ghuman, Fahad Majeed, Bebagar, Ahmed Khan, Muhammad Uzair, Ijaz Ahmed, Muhammad Ghani
Page Numbers : 122-132
DOI : 10.36348/sjls.2020.v05i07.002
Crops are very important in order to accommodate world population for food as well as industrial purposes. MiRNA (micro-RNA) have been considered as an important and crucial factor for manipulation of crops to make them more productive and resistant against harsh environmental stressed conditions. Now, these non-coding special sequences have been used successfully for gene-expression and regulation such as gene integration, slicing, signal transduction, pre and post translational modification, boost up metabolic pathways, enhancement of crop growth and developments and much more traits which is significant contribution in genetic engineering technologies for crop modifications. Genomic expression factors have been modulated through unique-miRNAs sequence which takes up toward the next generation specific targets that would have been adapted under biotechnological mechanisms and then these technologies could be used for improving the agronomic traits of various crops further down collection of high productive results. Significant strategies have pointed out to overcome the drawbacks during crop-manipulation. In this review, work for diversified and recently identified sequences of micro-RNA is studied; and production of valuable crops in order to have better agronomic properties to fulfill the requirements of food and industry with significant sustainability under stressed conditions. Genetic regulation as well as expression through miRNAs in genetic engineering will facilitate better for crop modification with quality and would be considered as positive step toward improving the economy of country.